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PEPTIDE SYNTHESIS In the organic chemistry, Peptide Synthesis is the production of peptides, which are organic compounds in which multiple amino acids are linked via amide bonds which are also known as Peptide Bonds. NH2 Automatic peptide synthesizer allows high quality synthesis of 0.1-1.0 mmol Surface resin- chemistry is the basis for automated NH2 NH2 peptides peptide synthesis 1880 1480- 1280- 1080 B80- 6во. 280 20 30 40 TIME (MIN) Precise high performance liquid chromatography allows purification of peptides for medical applications>99,9% Preparative scale high performance liquid chromatography allows fast purfication of large amount of peptides PEPTIDE SYNTHESIS CYCLE Resin Deprotetion Amino Acid PEPTIDE SYNTHESIS Cleavage CYCLE Activation Coupling Peptide BATCH SYNTHESIS Addition of solvent allows the resin to partially swell Limited emersion in solvent allows some reaction centres to be presented to the reagents In the dry form, the resin beads are closely packed, with the reaction centres 'hidden Incomplete acylation and/ or inaccessible reaction centres lead to truncated peptides Completion of the synthesis affords an array of truncated peptides bound to the resin REFERENCE PRESENTED BY: http://www.bluelionbio.com/labequip.phtml?mode= list&mode2=by_category&category_id=1o http://en.wikipedia.org/wiki/Peptide_synthesis http://www.ipfdd.de/fileadmin/user_upload/mbz/ mitarbeiter/freudenberg/peptide_synthesis.jpg http://moulder.temple.edu/sites/default/files/imce_uploads/fig9.gif http://www.pepceuticals.co.uk/media//Batch_Synthesis.jpg CWN OIE) nV